Regulatory
Part:BBa_K3425138
Designed by: Pedro Nunes Group: iGEM20_UofUppsala (2020-10-25)
Promoter ydfJ115
This promoter, native to B. subtilis, is positively regulated upon activation of the ydfHI two-component system through direct interaction with the DNA-binding domain of the response regulator ydfI. It is involved in the expression of ydfJ, a protein belonging to the RND superfamily. This promoter was used in the composite part BBa_K3425140 alongside a GFP.
References
Serizawa, M., and Sekiguchi, J. (2005) The Bacillus subtilis YdfHI two-component system regulates the transcription of ydfJ, a member of the RND superfamily. Microbiology. 151, 1769–1778
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//chassis/prokaryote/bsubtilis
//promoter
//promoter
chassis | B. subtilis |
function | Regulatory Promoter |
positive_regulators | ydfI |